Mouse IL-33 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-33 in the samples. A monoclonal antibody specific for mouse IL-33 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IL-33, and IL-33 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-33 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

8.74 pg/ml

Detektionsbereich

46.88 - 3000 pg/ml

Wiederherstellung

98% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse IL-33.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

C9orf26, CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, DVS27, DVS27 related protein, IL 1F11, IL 33, IL-1F11, IL1F11, IL33, IL33_HUMAN, Interleukin 1 family member 11, Interleukin 33, INTERLEUKIN 33 NFHEV, Interleukin 33 precursor, Interleukin-1 family member 11, Interleukin-33, Interleukin-33 (109-270), Interleukin33, NF HEV, NF-HEV, NFEHEV, NFHEV, Nuclear factor for high endothelial venules, Nuclear factor from high endothelial venules, OTTHUMP00000021041, RP11 575C20.2, RP11-575C20.2

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.