Maus IgG2a - BSA and Azide free - Isotype Control (A284002)

Mouse IgG2a - BSA and Azide free - Isotype Control

Flow Cytometry

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human T lymphocytes.

Mouse

Monoclonal

IgG2a

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product. THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.