Mouse sICAM-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse sICAM-1 in the samples. A monoclonal antibody specific for mouse sICAM-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for sICAM-1, and sICAM-1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sICAM-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

1.27 pg/ml

Detektionsbereich

125 - 8000 pg/ml

Wiederherstellung

102% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse sICAM-1.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.