Mouse CXCL9 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CXCL9 in the samples. A monoclonal antibody specific for mouse CXCL9 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CXCL9, and CXCL9 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL9 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

1.82 pg/ml

Detektionsbereich

15.63 - 1000 pg/ml

Wiederherstellung

98% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse CXCL9.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

C-X-C motif chemokine 9, Chemokine (C-X-C motif) ligand 9, Chemokine, CXC motif, ligand 9, CMK, Crg 10 , CXCL9_HUMAN, Gamma interferon induced monokine, Gamma-interferon-induced monokine, HuMIG, MIG, Monokine induced by gamma interferon, Monokine induced by interferon gamma, Monokine induced by interferon-gamma, SCYB9, Small inducible cytokine B9, Small inducible cytokine subfamily B member 9, Small-inducible cytokine B9

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.