Mouse MIP-2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse MIP-2 in the samples. A monoclonal antibody specific for mouse MIP-2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for MIP-2, and MIP-2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of MIP-2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

5.23 pg/ml

Detektionsbereich

7.81 - 500 pg/ml

Wiederherstellung

92% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse MIP-2.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

C-X-C motif chemokine 2, Chemokine (C X C motif) ligand 2, Chemokine (C-X-C motif) ligand 2, Chemokine, CXC motif, ligand 2, CINC 2a, CINC-2a, CINC2a, CINC3, CXC chemokine, CXCL 2, CXCL2_HUMAN, Cytokine-induced neutrophil chemoattractant 3, GRO 2, GRO b, Gro beta, GRO protein, beta, Gro-beta, GRO-beta(5-73), GRO-beta-T, GRO2, GRO2 oncogene, GROb, GRObeta, Growth regulated protein beta, Growth-regulated protein beta, GROX, Hematopoietic synergistic factor, HSF, Macrophage inflammatory protein 2, Macrophage inflammatory protein 2 alpha, Macrophage inflammatory protein 2-alpha, Melanoma growth stimulatory activity beta, MGSA b, MGSA beta, MGSA-b, MIP 2, MIP 2a, MIP2, MIP2 alpha, MIP2-alpha, MIP2A, MIP2alpha, SB-251353, Scyb, SCYB 2, SCYB2, Small inducible cytokine subfamily B, member 2

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.