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Maus CXCL16 Kit Elisa (A545)

$770
Versandkosten
Vorlaufzeit
Lieferung in 7-10 Werktagen
Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com
Produktname
Mouse CXCL16 ELISA Kit
Assay-Typ
Sandwich (quantitative)
Assay-Prinzip
This CXCL16 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-CXCL16 Antibody. Standards or samples are then added to the microtire plate wells and CXCL16, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of CXCL16 present in the sample, Anti-CXCL16 Antibody (Biotin) is added to each well to "sandwich" the CXCL16 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CXCL16 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensitivität
< 1 pg/ml
Detektionsbereich
15.6-1000 pg/ml
Reaktivität
Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
0910001K24Rik, AV290116, BB024863, C X C motif chemokine 16, C-X-C motif chemokine 16, Chemokine (C X C motif) ligand 16 , Chemokine, CXC motif, ligand 16, CXC chemokine ligand 16 , CXCLG16, CXL16_HUMAN, DN 491F10.6, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein , SCYB16, Small inducible cytokine B, Small inducible cytokine B16, Small inducible cytokine B16 precursor , Small-inducible cytokine B16, SR PSOX, SR PSOX/CXCL16 scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, SR-PSOX, SRPSOX, Transmembrane chemokine CXCL16 , UNQ2759/PRO6714, Zinc finger, MYND-type containing 15, Zmynd15
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

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