Mouse RANTES ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse RANTES in the samples. A monoclonal antibody specific for mouse RANTES has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for RANTES, and RANTES present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of RANTES bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.12 pg/ml

Detektionsbereich

15.63 - 1000 pg/ml

Wiederherstellung

96% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse RANTES.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

Beta chemokine RANTES, Beta chemokine RANTES precursor, C C motif chemokine 5, CCL 5, CCL5_HUMAN, Chemokine (C C motif) ligand 5, Chemokine CC Motif Ligand 5, D17S136E, EoCP, Eosinophil chemotactic cytokine, MGC17164, RANTES, RANTES(4-68), Regulated upon activation normally T expressed and presumably secreted, SCYA 5, SCYA5, SIS delta, SIS-delta, SISd, Small inducible cytokine A5, Small inducible cytokine A5 (RANTES), Small inducible cytokine subfamily A (Cys Cys) member 5, Small-inducible cytokine A5, T cell specific protein p288 , T cell specific RANTES protein, T cell-specific protein P228, T-cell-specific protein RANTES, TCP 228, TCP228

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.