Mouse CCL22 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse CCL22 in the samples. A monoclonal antibody specific for mouse CCL22 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CCL22, and CCL22 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL22 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

4.07 pg/ml

Detektionsbereich

31.25 - 2000 pg/ml

Wiederherstellung

84% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse CCL22.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

A 152E5.1, A-152E5.1 , ABCD 1, ABCD1, C C motif chemokine 22, C-C motif chemokine 22, CC chemokine STCP 1, CC chemokine STCP-1, ccl 22, CCL22_HUMAN, Chemokine (C C motif) ligand 22, Chemokine (C-C motif) ligand 22, DC/B CK, DC/B-CK, DCBCK, Macrophage-derived chemokine, MDC, MDC(1-69), MDC(7-69), MGC34554, SCYA22, Small inducible cytokine A22, Small inducible cytokine subfamily A (Cys Cys) member 22, Small inducible cytokine subfamily A (Cys-Cys), member 22, Small inducible cytokine subfamily A, member 22, Small-inducible cytokine A22, STCP 1, STCP1, Stimulated T cell chemotactic protein 1, Stimulated T-cell chemotactic protein 1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.