Mouse CCL11 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse Eotaxin in the samples. A monoclonal antibody specific for mouse Eotaxin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Eotaxin, and Eotaxin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Eotaxin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.73 pg/ml

Detektionsbereich

15.63 - 1000 pg/ml

Wiederherstellung

97% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse Eotaxin.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

C-C motif chemokine 11, CCL 11 , CCL11_HUMAN, Chemokine (C-C motif) ligand 11, Chemokine C C motif ligand 11, Chemokine CC Motif Ligand 11, Chemokine ligand 11, Eosinophil Chemotactic P, Eosinophil chemotactic protein, Eotaxin, Eotaxin 1, eotaxin-1, MGC22554, SCYA 11 , SCYA11 , Small inducible cytokine A11 , Small inducible cytokine subfamily A (Cys Cys) member 11 , small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin), Small inducible cytokine subfamily A member 11 , Small-inducible cytokine A11

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.