Anti-MMP13 Antibody (A92641)

Name: Anti-MMP13 Antibody
Brand: Antibodies.com
SKU: A92641

Enlarge Image

This product is discontinued

Anti-MMP13 Antibody (A92641) has been discontinued and is no longer available.

View all MMP13 Antibodies.

Specifications

Name

Anti-MMP13 Antibody

Description

Rabbit polyclonal antibody to MMP13.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 104-272 of human MMP13 (NP_002418.1).

Sequence

YNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDED

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa / 55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Collagenase 3, Matrix metalloproteinase-13, MMP-13

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

Enlarge Image

Western Blot - Anti-MMP13 Antibody (A92641)

Western blot analysis of extracts of various cell lines, using Anti-MMP13 Antibody (A92641) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Western Blot - Anti-MMP13 Antibody (A92641)

Western blot analysis of extracts from normal (control) and MMP13 knockout (KO) 293T cells, using Anti-MMP13 Antibody (A92641) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Immunofluorescence - Anti-MMP13 Antibody (A92641)

Immunofluorescence analysis of rat trachea using Anti-MMP13 Antibody (A92641) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).