Figure 1: Western Blot - Anti-MLH3 Antibody (A10222)
Western blot analysis of HCT116, using Anti-MLH3 Antibody (A10222) at 1:3,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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