Anti-Mitofusin 2 Antikörper [S153-5] (A304787)

Name: Anti-Mitofusin 2 Antibody [S153-5]
Brand: Antibodies.com
SKU: A304787
Price: 500.0 USD
Availability: InStock

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$500

Monoklonal [S153-5] Maus Antikörper gegen Mitofusin 2 für WB, IHC und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Polyklonal Kaninchen Antikörper gegen Mitofusin 2 für ELISA, IHC, IP und WB.

Produktdetails

Produktname

Anti-Mitofusin 2 Antibody [S153-5]

Beschreibung

Mouse monoclonal [S153-5] antibody to Mitofusin 2.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:1,000, ICC/IF: 1:100

Reaktivität

Human, Mouse, Rat

Kreuzreaktivität

This antibody does not cross-react with Mitofusin-1.

Immunogen

Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1..

Wirt

Mouse

Klonalität

Monoclonal

Klon

S153-5

Isotyp

IgG2a

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

~90 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

CMT2A, CMT2A2, CPRP 1, CPRP1, EC 3.6.5.-, Fzo, HSG, hyperplasia suppressor gene, Hypertension related protein 1, KIAA0214, MARF, MFN 2, Mfn2, MFN2_HUMAN, Mitochondrial assembly regulatory factor, Mitofusin-2, Mitofusin2, Transmembrane GTPase MFN2

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Immunofluorescence - Anti-Mitofusin 2 Antibody [S153-5] (A304787)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Mitofusin 2 Antibody [S153-5] (A304787), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Mitofusin 2 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-Mitofusin 2 Antibody [S153-5] (A304787)

Western blot analysis of rat brain membrane showing detection of ~90 kDa Mitofusin 2 protein using Anti-Mitofusin 2 Antibody [S153-5] (A304787) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~90 kDa.

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Figure 3: Immunofluorescence - Anti-Mitofusin 2 Antibody [S153-5] (A304787)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Mitofusin 2 Antibody [S153-5] (A304787), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Mitofusin 2 Antibody. (D) Composite.