Anti-MIF Antikörper [MIF/3488] (A277706)

Name: Anti-MIF Antibody [MIF/3488]
Brand: Antibodies.com
SKU: A277706
Price: 525.0 USD
Availability: InStock

Bild Vergrößern

$525

Monoklonal [MIF/3488] Maus Antikörper gegen MIF für WB und IHC-P.

100% Qualitätsgarantie

Preis-Match-Garantie

Alternative Formats and ConjugatesAlternative Formats

Carrier Free and Preservative Free

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 4-6 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Stichworte:

Monospezifischer Antikörper

Produktdetails

Produktname

Anti-MIF Antibody [MIF/3488]

Beschreibung

Mouse monoclonal [MIF/3488] antibody to MIF.

Spezifität

Macrophage migration inhibitory factor, known as MIF or glycosylationinhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor Î± (TNFÎ±), interleukin-1 (IL-1) and interferon-Î³ (IFN-Î³). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

Anwendungen

WB, IHC-P

Verdünnungen

WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Reaktivität

Human

Immunogen

Recombinant fragment corresponding to human MIF protein. The exact sequence is proprietary.

Wirt

Mouse

Klonalität

Monoclonal

Klon

MIF/3488

Isotyp

IgG2a

Leichte Ketten

kappa

Konjugat

Unconjugated

Reinigung

Protein A/G chromatography.

Konzentration

200 µg/ml

Molekulargewicht

13 kDa

Produktform

Liquid

Formulierung

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Allgemeine Hinweise

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-MIF Antibody [MIF/3488] - BSA and Azide free (A278294).

Synonyme

GIF, GLIF, Glycosylation inhibiting factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Macrophage migration inhibitory factor, Macrophage migration inhibitory factor (glycosylation-inhibiting factor), MIF protein, MIF_HUMAN, MMIF, Phenylpyruvate tautomerase

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

Bild Vergrößern

Figure 1: Immunohistochemistry - Anti-MIF Antibody [MIF/3488] (A277706)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue using Anti-MIF Antibody [MIF/3488]. Inset: PBS instead of the primary antibody. Secondary antibody negative control.

Bild Vergrößern

Figure 2: SDS-PAGE - Anti-MIF Antibody [MIF/3488] (A277706)

SDS-PAGE analysis of Anti-MIF Antibody [MIF/3488] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

Bild Vergrößern

Figure 3: Western Blot - Anti-MIF Antibody [MIF/3488] (A277706)

Western blot analysis of (Lane 1) LNCaP cell line lysate and (Lane 2) PC3 cell line lysate using Anti-MIF Antibody [MIF/3488].

Bild Vergrößern

Figure 4: Anti-MIF Antibody [MIF/3488] (A277706)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-MIF Antibody [MIF/3488]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.