This antibody recognises macrophage migration inhibitory factor (MIF), also known referred to as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase. MIF is an important regulator of innate immunity. Bacterial antigens stimulate white blood cells to release MIF into the blood stream. The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. Hence, MIF is classified as an inflammatory cytokine
Applications
WB, IP
Dilutions
IP: 5 - 10 µg/ml
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues surrounding amino acid 67 of human MIF.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
200 µg/ml
Molecular Weight
Approximately 12 kDa in Jurkat and rat kidney lysates
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 30% Glycerol, 0.5% BSA, 0.01% Thiomersal, and 5mM EDTA.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti MIF antibody recognizes macrophage migration inhibitory factor (MIF), also known referred to asglycosylation-inhibiting factor(GIF),L-dopachrome isomerase, or phenylpyruvate tautomerase.MIF is an important regulator of innate immunity. Bacterial antigens stimulate white blood cellsto release MIF into the blood stream.The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. Hence, MIF is classified as an inflammatorycytokine