Anti-MHC Class I Antibody [VPM19] (A281931)

Anti-MHC Class I Antibody [VPM19]

Mouse monoclonal [VPM19] antibody to MHC Class I.

This antibody recognises the ovine homologue of the human MHC Class I, a monomorphic determinant expressed on the heavy chain of sheep MHC Class I, (OLA Class I).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In sheep, this is often referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC Class I functions in the recognition and presentation of foreign antigens to T-cells.Ovine MHC Class I is a membrane glycoprotein with a molecular weight of approximately 44kDa, expressed on the cell surface of all peripheral blood leucocytes.Clone VPM19 has been in used in a number of domestic animal disease states, in particular Maedi Visna virus infection, a disease of significant importance in commercial sheep flocks (Lee et al. 1996, Ryan et. al. 2000 and Wu et. al. 2008). recognises MHC class I in other species and has been used in a study of feline herpes virus infection (Montagnaro et. al. 2009).

WB, IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Sheep, Cat

Sheep T cells.

Mouse

Monoclonal

VPM19

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Sheep MHC Class I antibody, clone VPM19 recognizes the ovine homologue of the human MHC Class I, a monomorphic determinant expressed on the heavy chain of sheep MHC Class I, (OLA Class I).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In sheep, this is often referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC Class I functions in the recognition and presentation of foreign antigens to T-cells.Ovine MHC Class I is a membrane glycoprotein with a molecular weight of approximately 44kDa, expressed on the cell surface of all peripheral blood leucocytes.Clone VPM19 has been in used in a number of domestic animal disease states, in particular Maedi Visna virus infection, a disease of significant importance in commercial sheep flocks (Lee et al. 1996, Ryan et. al. 2000 and Wu et. al. 2008). Mouse anti Sheep MHC Class I antibody, clone VPM19 recognizes MHC class I in other species and has been used in a study of feline herpes virus infection (Montagnaro et. al. 2009).