Anti-MEK1 (phospho Ser298) Antikörper (A16425)

Name: Anti-MEK1 (phospho Ser298) Antibody
Brand: Antibodies.com
SKU: A16425
Price: 435.0 USD
Availability: InStock

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$435

Polyklonal Kaninchen Antikörper gegen MEK1 (phospho Ser298) für WB und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-MEK1 (phospho Ser298) Antibody

Beschreibung

Rabbit polyclonal antibody to MEK1 (phospho Ser298).

Anwendungen

WB, IP

Verdünnungen

WB: 1:500-1:2,000, IP: 1:50-1:100

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S298 of human MEK1 (NP_002746.1).

Sequenz

PLSSY

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

43 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

Dual specificity mitogen activated protein kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP kinase/Erk kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEKK1, Mitogen activated protein kinase kinase 1, MKK 1, MKK1, MP2K1_HUMAN, PRKMK1, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1), Protein kinase mitogen activated, kinase 1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-MEK1 (phospho Ser298) Antibody (A16425)

Western blot analysis of extracts of various cell lines, using Anti-MEK1 (phospho Ser298) Antibody (A16425) at 1:1,000 dilution or MEK1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-MEK1 (phospho Ser298) Antibody (A16425)

Western blot analysis of extracts of various cell lines, using Anti-MEK1 (phospho Ser298) Antibody (A16425) at 1:1,000 dilution. C2C12 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by ATP(5 mM) at 30°C for 1 hour. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour or treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-MEK1 (phospho Ser298) Antibody (A16425)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-MEK1 (phospho Ser298) Antibody (A16425). This Western blot was performed on the immunoprecipitate using Anti-MEK1 (phospho Ser298) Antibody (A16425) at a dilution of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.