MED26 Cell Based ELISA Kit (A103467)

MED26 Cell Based ELISA Kit

Cell-based (qualitative)

This MED26 Cell Based ELISA Kit allows for the detection of MED26 and the effects that certain stimulation conditions have on MED26 expression in different cell lines. Qualitative determination of MED26 concentration is achieved by an indirect ELISA format. In essence, the MED26 is captured by Anti-MED26 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this MED26 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

Activator-recruited cofactor 70 kDa component, ARC70, Cofactor required for Sp1 transcriptional activation subunit 7, CRSP complex subunit 7, CRSP7, Mediator complex subunit 26, Mediator of RNA polymerase II transcription subunit 26, Transcriptional coactivator CRSP70