Western blot analysis of extracts of NIH/3T3 cells, using Anti-MDM2 Antibody (A12616) at 1:1,000 dilution. NIH/3T3 cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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