Anti-MDA5 Antibody (A83931)

Name: Anti-MDA5 Antibody
Brand: Antibodies.com
SKU: A83931
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to MDA5 for ELISA, WB, IHC and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 3-5 business days.

Specifications

Name

Anti-MDA5 Antibody

Description

Goat polyclonal antibody to MDA5.

Applications

ELISA, WB, IHC, Flow Cytometry

Dilutions

ELISA: 1:32,000, IHC: 10ug/ml, Flow Cytometry: 10µg/ml

Reactivity

Human, Monkey

Immunogen

Synthetic peptide corresponding to the N terminal region of human MDA5.

Sequence

SNGYSTDENFRYL-C

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CADM-140 autoantigen, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Helicard, Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, Interferon-induced with helicase C domain protein 1, MDA-5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Immunohistochemistry - Anti-MDA5 Antibody (A83931)

MDA5 expression in Human Pancreas analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in Tris/EDTA buffer, pH 9. Staining was performed with Anti-MDA5 Antibody (A83931) at 10µg/ml and revealed with horseradish peroxidase (HRP).

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Flow Cytometry - Anti-MDA5 Antibody (A83931)

MDA5 expression in K562 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-MDA5 Antibody (A83931) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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