Mouse monoclonal [MAC387] antibody to Macrophages/Monocytes/Granulocytes (PE).
Spezifität
This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.
Anwendungen
Flow Cytometry
Verdünnungen
FC 1: Neat - 1:5, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Protein A affinity chromatography of tissue culture supernatant.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with 1 ml of distilled water.
Formulierung
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Lagerung
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Allgemeine Hinweise
Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.
