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Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin) (A320888)

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Secondary Structure - Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin) (A320888) - Antibodies.com
$730
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Name
Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin)
Description
Synthetic DNA aptamer to Lysozyme [Part of Lysozyme Aptasensor] (Biotin).
Specificity
BSA was used as a negative control.
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
NE Buffer 2 (10X): 10mM Tris-HCl Buffer, pH 7.9, with 50mM Sodium Chloride, 10mM Magnesium Chloride, and 1mM Dithiothreitol. Detection Buffer: 25mM HEPES Buffer, pH 7.4, with 20mM Potassium Chloride, 200mM Sodium Chloride, 0.05% Triton X-100, 1% DMSO, and 0.5µM Hemin. Binding Temperature: 25°C.
Sequence
5'-dApdTpdCpdTpdApdCpdGpdApdApdTpdTpdCpdApdTpdCpdApdGpdGpdGpdCpdTpdApdApdApdGpdApdGpdTpdGpdCpdApdGpdApdGpdTpdTpdApdCpdTpdTpdApdGpdCpdCpdCpdGpdTpdGpdAp-3'
Aptamer Length
49 nt
Aptamer Molecular Weight (Da)
15,128.91 Da
Aptamer Affinity
LOD = 0.1 fM
Extinction Coefficient
488200 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin) (A320888) is a replica of the aptamer described in PMID: 21826311.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Secondary Structure - Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin) (A320888) - Antibodies.comBild Vergrößern
Figure 1: Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin) (A320888)
Secondary structure of Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin) (A320888), with a dG of -4.97 kcal/mol.
PubMed LogoPublikation anzeigen
An ultrasensitive peroxidase DNAzyme-associated aptasensor that utilizes a target-triggered enzymatic signal amplification strategy
Verweise: Anti-Lysozyme Aptamer [Part of Lysozyme Aptasensor] (Biotin) (A320888)
Abstrakt:

By rationally employing enzymes for signal amplification, an ultrasensitive aptasensor was developed and successfully tested in a system designed to detect lysozyme with a detection limit of 0.1 fM. This detection limit is nearly three orders of magnitude lower than those of any previously reported DNAzyme-based aptasensors.

Publikation anzeigen
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