Anti-Leptin Receptor Antikörper (A83422)

Name: Anti-Leptin Receptor Antibody
Brand: Antibodies.com
SKU: A83422
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab50424). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen Leptin Receptor für ELISA, WB, IHC und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-Leptin Receptor Antibody

Beschreibung

Goat polyclonal antibody to Leptin Receptor.

Anwendungen

ELISA, WB, IHC, Flow Cytometry

Verdünnungen

ELISA: 1:128,000, WB: 1-2µg/ml, IHC: 4-6µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the internal region of human Leptin Receptor.

Sequenz

C-TQDDIEKHQSDAG

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

140-150 kDa

Geschätztes MW

132kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

CD 295, CD295, CD295 antigen, Db, Fa, HuB219, LEP R, LEP-R, LEPR, LEPRD, LEPR_HUMAN, Leptin receptor fatty, Leptin receptor gene related protein, Leptin receptor precursor, OB R gene related protein, OB receptor, OB-R, OB-RGRP, obl, Obr

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Western Blot - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in Human Cerebellum (A), Mouse Foetal Brain (B), and Rat Brain (C) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Leptin Receptor Antibody (A83422) at 1.75µg/ml (A) or 1.5µg/ml (B-C) and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Leptin Receptor Antibody (A83422) at 6µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 3: Immunohistochemistry - Anti-Leptin Receptor Antibody (A83422)

Negative control for Leptin Receptor expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 4: Flow Cytometry - Anti-Leptin Receptor Antibody (A83422)

Leptin Receptor expression in K562 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Leptin Receptor Antibody (A83422) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.