Anti-Lamin B2 Antibody [ARC1252] (A309214)

Name: Anti-Lamin B2 Antibody [ARC1252]
Brand: Antibodies.com
SKU: A309214
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1252] antibody to Lamin B2 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Lamin B2 Antibody [ARC1252]

Description

Rabbit monoclonal [ARC1252] antibody to Lamin B2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 521-620 of human Lamin B2 (Q03252).

Sequence

YILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1252

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Lamin-B2, LMN2, LMNB2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Lamin B2 Antibody [ARC1252] (A309214)

Western blot analysis of extracts of various cell lines, using Anti-Lamin B2 Antibody [ARC1252] (A309214) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Lamin B2 Antibody [ARC1252] (A309214)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Lamin B2 Antibody [ARC1252] (A309214) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Lamin B2 Antibody [ARC1252] (A309214)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Lamin B2 Antibody [ARC1252] (A309214) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Lamin B2 Antibody [ARC1252] (A309214)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Lamin B2 Antibody [ARC1252] (A309214) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.