Anti-Ku70 Antibody [KU729] - BSA and Azide free (A251855)

Name: Anti-Ku70 Antibody [KU729] - BSA and Azide free
Brand: Antibodies.com
SKU: A251855
Price: 545.0 USD
Availability: InStock

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$545

Mouse monoclonal [KU729] antibody to Ku70 for Flow Cytometry, IF and ICC.

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Alternative Formats and ConjugatesAlternative Formats

PE Unconjugated

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Ku70 Antibody [KU729] - BSA and Azide free

Description

Mouse monoclonal [KU729] antibody to Ku70.

Specificity

This antibody recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

Applications

Flow Cytometry, IF, ICC

Dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, ICC: 1-2 µg/ml

Reactivity

Human

Cross Reactivity

This antibody is predicted to cross react with Mouse, Rat, or Chicken.

Immunogen

Nuclear extract of human HL-60 cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

KU729

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Molecular Weight

70 kDa / 80 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Ku70 Antibody [KU729] (A248673).

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Flow Cytometry - Anti-Ku70 Antibody [KU729] - BSA and Azide free (A251855)

Flow cytometric analysis of K562 cells labeling Ku70 with Anti-Ku70 Antibody [KU729] (PE) (Red). Cells alone (Black). Isotype Control (Green).

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Flow Cytometry - Anti-Ku70 Antibody [KU729] - BSA and Azide free (A251855)

Flow cytometric analysis of 293T cells labeling Ku70 with Anti-Ku70 Antibody [KU729] (AF488) (Green). Cells alone (Black). Isotype Control (Grey).

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Immunofluorescence - Anti-Ku70 Antibody [KU729] - BSA and Azide free (A251855)

Immunofluorescent analysis of paraformaldehyde fixed HeLa cells stained with Anti-Ku70 Antibody [KU729] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin (Red).

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SDS-PAGE - Anti-Ku70 Antibody [KU729] - BSA and Azide free (A251855)

SDS-PAGE analysis of Anti-Ku70 Antibody [KU729] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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