Western Blot - Anti-K27-linkage specific Polyubiquitin Antibody (A309527)
Western blot analysis of extracts of various cell lines, using Anti-K27-linkage specific Polyubiquitin Antibody (A309527) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Dot Blot - Anti-K27-linkage specific Polyubiquitin Antibody (A309527)
Dot blot analysis of a mixture of peptides using Anti-K27-linkage specific Polyubiquitin Antibody (A309527) at a 1:1,000 dilution.
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