Figure 1: Western Blot - Anti-JunD Antibody [ARC1409] (A307304)
Western blot analysis of extracts of various cell lines, using Anti-JunD Antibody [ARC1409] (A307304) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-JunD Antibody [ARC1409] (A307304) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-JunD Antibody [ARC1409] (A307304) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-JunD Antibody [ARC1409] (A307304) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Figure 5: Western Blot - Anti-JunD Antibody [ARC1409] (A307304)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-JunD Antibody [ARC1409] (A307304). This Western blot was performed on the immunoprecipitate using Anti-JunD Antibody [ARC1409] (A307304) at a dilution of 1:1000.