Anti-ISG15 Antibody (A11423)

Western blot analysis of extracts of various cell lines, using Anti-ISG15 Antibody (A11423) at 1:1,000 dilution. Jurkat cells were treated by IFN-alpha (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-ISG15 Antibody (A11423) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-ISG15 Antibody (A11423) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.