Figure 1: Western Blot - Anti-IRS1 (phospho Ser1101) Antibody (A306239)
Western blot analysis of extracts of C2C12 cells, using Anti-IRS1 (phospho Ser1101) Antibody (A306239) at 1:1,000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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