This antibody recognises human interferon regulatory factor 5 (IRF5), a ~58 kDa member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells.The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10.

Anwendungen

ELISA, WB, IHC-P, IP

Verdünnungen

IHC-P 1: 1:50, WB: 1:1,000 - 1:2,000

Reaktivität

Human

Immunogen

Histidine-tagged recombinant peptide corresponding to amino acids 176-240 of human IRF5, purified from E. coli.

Sequenz

PPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLI

Wirt

Mouse

Klonalität

Monoclonal

Klon

10T1

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein A affinity chromatography of ascites.

Konzentration

1 mg/ml

Molekulargewicht

Approximately 58 kDa in THP-1 cell lysates.

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 10% Glycerol and 0.02% Sodium Azide.

Lagerung

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Allgemeine Hinweise

Mouse anti Human IRF5 antibody, clone 10T1 recognizes human interferon regulatory factor 5 (IRF5), a ~58 kDa member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells.The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10 (Barnes et al. 2002).

Synonyme

Interferon regulatory factor 5, Interferon regulatory factor 5 bone marrow variant, IRF 5, IRF-5, IRF5_HUMAN, SLEB10

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.