Anti-IRF4 Antikörper (A286064)

Name: Anti-IRF4 Antibody
Brand: Antibodies.com
SKU: A286064
Price: 480.0 USD
Availability: InStock

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$480

Polyklonal Ziege Antikörper gegen IRF4 für ELISA, WB, IHC und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-IRF4 Antibody

Beschreibung

Goat polyclonal antibody to IRF4.

Anwendungen

ELISA, WB, IHC, Flow Cytometry

Verdünnungen

ELISA: 1:16,000, WB: 0.1-0.3µg/ml, IHC: 5µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human, Rat

Immunogen

Synthetic peptide corresponding to the internal region (near C terminus), amino acids 393-407, of human IRF4.

Sequenz

C-DPQRQRKLITAHVEP

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

55 kDa

Geschätztes MW

51.8kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

Interferon regulatory factor 4, IRF 4, IRF-4, IRF4_HUMAN, LSIRF, Lymphocyte specific interferon regulatory factor, Lymphocyte specific IRF, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1, MUM 1, MUM1, NF EM5, NF-EM5, NFEM5, PU.1 interaction partner, Sfpi1/PU.1 interaction partner, Transcriptional activator PIP

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-IRF4 Antibody (A286064)

IRF4 expression in Human (A) and Rat (B) Spleen lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-IRF4 Antibody (A286064) at 0.3µg/ml (A) or 0.1µg/ml (B).

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Figure 2: Immunohistochemistry - Anti-IRF4 Antibody (A286064)

IRF4 expression in Human Spleen analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-IRF4 Antibody (A286064) at 5µg/ml and revealed with alkaline phosphatase (AP).

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Figure 3: Flow Cytometry - Anti-IRF4 Antibody (A286064)

IRF4 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IRF4 Antibody (A286064) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.