Anti-IP3 receptor Antibody (A308643)

Name: Anti-IP3 receptor Antibody
Brand: Antibodies.com
SKU: A308643

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This product is discontinued

Anti-IP3 receptor Antibody (A308643) has been discontinued and is no longer available.

View all IP3 receptor Antibodies.

Specifications

Name

Anti-IP3 receptor Antibody

Description

Rabbit polyclonal antibody to IP3 receptor.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1850-1950 of human IP3R (XP_011531985.1).

Sequence

SFFCRLTEDKKSEKFFKVFYDRMKVAQQEIKATVTVNTSDLGNKKKDDEVDRDAPSRKKAKEPTTQITEEVRDQLLEASAATRKAFTTFRREADPDDHYQP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1, Inositol 1,4,5-trisphosphate-gated calcium channel ITPR1, INSP3R1, IP3 receptor isoform 1, IP3R 1, ITPR1, Type 1 inositol 1,4,5-trisphosphate receptor, Type 1 InsP3 receptor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-IP3 receptor Antibody (A308643)

Western blot analysis of extracts of Mouse brain, using Anti-IP3 receptor Antibody (A308643) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-IP3 receptor Antibody (A308643)

Western blot analysis of extracts of various cell lines, using Anti-IP3 receptor Antibody (A308643) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Immunofluorescence - Anti-IP3 receptor Antibody (A308643)

Immunofluorescence analysis of U-2 OS cells using Anti-IP3 receptor Antibody (A308643) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue). Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.