Anti-Integrin alpha V Antikörper (A13861)

Western blot analysis of extracts of various cell lines, using Anti-Integrin alpha V Antibody (A13861) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts of MCF7 cells, using Anti-Integrin alpha V Antibody (A13861) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Integrin alpha V Antibody (A13861) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 200µg extracts of C6 cells using 3µg of Anti-Integrin alpha V Antibody (A13861). This Western blot was performed on the immunoprecipitate using Anti-Integrin alpha V Antibody (A13861) at a dilution of 1:2000.