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Anti-Influenza-B-Virus Hemagglutinin Antikörper [10B8] (A338)

Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control. g. 2 Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 10B8 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 10B8 recognizes an epitope on HA2
$535

Monoklonal [10B8] Maus Antikörper gegen Influenza B Virus Hemagglutinin für WB, IP, ICC/IF und ELISA.

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Versandkosten
$40
Vorlaufzeit
Lieferung in 4-6 Werktagen

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Produktname
Anti-Influenza B Virus Hemagglutinin Antibody [10B8]
Beschreibung
Mouse monoclonal (10B8) antibody to Influenza B Virus Hemagglutinin.
Anwendungen
WB, IP, ICC/IF, ELISA
Verdünnungen
WB: 1:500-1:1,000, ICC/IF: 1:100-1:200, IP: 1:200
Reaktivität
Influenza B Virus
Kreuzreaktivität
This antibody does not react with Influenza A viruses.
Immunogen
Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group.
Wirt
Mouse
Klonalität
Monoclonal
Klon
10B8
Isotyp
IgG2a
Konjugat

Unconjugated

Reinigung
Affinity purification.
Konzentration
1 mg/ml
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.Bild Vergrößern
Figure 1: Immunofluorescence - Anti-Influenza B Virus Hemagglutinin Antibody [10B8] (65-165)
Anti-Influenza B Virus Hemagglutinin Antibody
g. 2 Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 10B8 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 10B8 recognizes an epitope on HA2Bild Vergrößern
Figure 2: Anti-Influenza B Virus Hemagglutinin Antibody [10B8] (65-165)
Anti-Influenza B Virus Hemagglutinin Antibody

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Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region..
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Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. Shandong/7/97 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region..
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