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Anti-Influenza-A-Virus Nucleoprotein Antikörper [C43] (A335)

Dieses Produkt ist identisch mit Abcam (ab128193). Entdecken Sie mehr für weniger.

Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HA Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses. Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.
$535

Monoklonal [C43] Maus Antikörper gegen Influenza A Virus Nucleoprotein für WB, IP, IF, IHC und ELISA.

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Produktname
Anti-Influenza A Virus Nucleoprotein Antibody [C43]
Beschreibung
Mouse monoclonal (C43) antibody to Influenza A Virus Nucleoprotein.
Spezifität
This antibody reacts with NP of all Influenza A viruses tested so far, including seasonal H2N2, H3N2, and avian H5N1, H5N2, and H1N1 (seasonal, pandemic, and swine).
Anwendungen
WB, IP, IF, IHC, ELISA
Verdünnungen
WB: 1:300-1:1,000, IP: 1:100, IF: 1:200, IHC: 1:200
Reaktivität
Influenza A Virus
Kreuzreaktivität
This antibody does not react with Influenza B viruses.
Immunogen
Human Influenza A Virus (H2N2) Okada strain.
Wirt
Mouse
Klonalität
Monoclonal
Klon
C43
Isotyp
IgG2a
Konjugat

Unconjugated

Reinigung
Affinity purification.
Konzentration
1 mg/ml
Reinheit
> 90% (by SDS-PAGE)
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibodyBild Vergrößern
Figure 1: Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HABild Vergrößern
Figure 2: Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses.Bild Vergrößern
Figure 3: Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody
Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.Bild Vergrößern
Figure 4: Anti-Influenza A Virus Nucleoprotein Antibody [C43] (65-110)
Anti-Influenza A Virus Nucleoprotein Antibody

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