This antibody recognises human IL-33 (interleukin-33), formerly known as NF-HEV (nuclear factor from high endothelial venules), a recently described member of the IL-1 superfamily which signals through interaction with the ST2 and Toll-interleukin 1 (IL-1) receptors, and is known to act as an immunoregulator of T helper 2 (Th2) cell cytokine synthesis.
Applications
WB, ELISA
Dilutions
ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml
Reactivity
Human
Immunogen
Recombinant human IL-33.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Biotin
Concentration
100 µg/ml after reconstituiton.
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti Human Interleukin-33 antibody recognizes human IL-33 (interleukin-33), formerly known as NF-HEV (nuclear factor from high endothelial venules), a recently described member of the IL-1 superfamily which signals through interaction with the ST2 and Toll-interleukin 1 (IL-1) receptors, and is known to act as an immunoregulator of T helper 2 (Th2) cell cytokine synthesis.
Synonyms
C9orf26, IL-1F11, IL1F11, IL33, Interleukin-1 family member 11, Interleukin-33, NF-HEV, NFHEV, Nuclear factor from high endothelial venules
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-IL-33 Antibody (Biotin) - BSA and Azide free (A282259)? Please let us know so that we can list the citation on this page.