Figure 1: Western Blot - Anti-IL-2 Antibody [ARC5130-01] (A310010)
Western blot analysis of extracts of 293T cells, using Anti-IL-2 Antibody [ARC5130-01] (A310010) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 2: Western Blot - Anti-IL-2 Antibody [ARC5130-01] (A310010)
Western blot analysis of extracts of Jurkat cells, using Anti-IL-2 Antibody [ARC5130-01] (A310010) at 1:1,000 dilution. Jurkat cells were treated by TPA (40 nM), A23187 (2µM) and Brefeldin A (300 ng / ml) for 0-24hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
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