Western blot analysis of extracts of Jurkat cells, using Anti-IL-2 Antibody (A93243) at 1:1,000 dilution. Jurkat cells were treated by TPA (40 nM), A23187 (2µM) and Brefeldin A (300 ng / ml) for 0-24hoursThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of Recombinant Human IL2 Protein, using Anti-IL-2 Antibody (A93243) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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