This antibody recognises mouse Interleukin-13 (IL-13), a 12.3kDa immunoregulatory cytokine produced primarily by activated T helper type 2 (Th2) cells and also by mast cells and NK cells.The biological properties of IL-13 include stimulation of B cell proliferation and antibody production. IL-13 plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E and eosinophils.
Anwendungen
WB, ELISA
Verdünnungen
ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml
Reaktivität
Mouse
Immunogen
Recombinant mouse IL-13.
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat
Biotin
Konzentration
100 µg/ml after reconstitution.
Produktform
Lyophilized
Rekonstitution
Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Formulierung
Supplied in Phosphate Buffered Saline.
Lagerung
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Allgemeine Hinweise
Rabbit anti Mouse Interleukin-13 antibody recognizes mouse Interleukin-13 (IL-13), a 12.3kDa immunoregulatory cytokine produced primarily by activated T helper type 2 (Th2) cells and also by mast cells and NK cells.The biological properties of IL-13 include stimulation of B cell proliferation and antibody production. IL-13 plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E and eosinophils (Wills-Karp et al. 1998).
