This IKB alpha (phospho Tyr42) Cell Based ELISA Kit allows for the detection of IKB alpha (phospho Tyr42) and the effects that certain stimulation conditions have on IKB alpha (phospho Tyr42) expression in different cell lines. Qualitative determination of IKB alpha (phospho Tyr42) concentration is achieved by an indirect ELISA format. In essence, the IKB alpha (phospho Tyr42) is captured by Anti-IKB alpha (phospho Tyr42) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this IKB alpha (phospho Tyr42) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-IKB alpha Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Adherent cells and suspension cells.

Detektionsbereich

> 5000 Cells

Testdauer

4 hours 30 minutes

Reaktivität

Human, Mouse, Rat

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at + 4°C. Stable for 6 months.

Synonyms

I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, IKBA, IKBalpha, IKBA_HUMAN, MAD 3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.