Anti-Ikaros Antibody [4E9] (A278877)

Anti-Ikaros Antibody [4E9]

Mouse monoclonal [4E9] antibody to Ikaros.

This antibody recognises Ikaros also known as Ikaros family zinc finger protein 1 (IFZF1) a DNA binding protein. It is expressed by hematopoietic cells, lymphoid cells and some subpopulations of myeloid cells. Ikaros is required for lymphocyte development and mediates B and T cell differentiation. It is thought that Ikaros regulates these processes by altering transcription of its target genes through chromatin remodeling of DNA promoter regions. To regulate transcription, Ikaros forms homo- or heterodimers with other Ikaros family members such as Helios, Aiolos, Eos or Pegasus. This dimerization forms a complex which is capable of binding DNA and initiating its transcriptional activity (Merkenschlager 2010). This clone 4E9 has been used in immunocytochemistry experiments to examine Ikaros expression in cells incubated with cholesterol to examine how the transcription factor modulates cholesterol uptake.

Flow Cytometry, IP, WB, ICC

Human, Mouse

Recombinant human Ikaros (C-terminus).

Mouse

Monoclonal

4E9

IgG1

Protein A affinity chromatography of tissue culture supernatant.

>95% (by SDS-PAGE).

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Store undiluted at 4°C. Do not freeze!

Mouse anti Human Ikaros, clone 4E9 recognizes Ikaros also known as Ikaros family zinc finger protein 1 (IFZF1) a DNA binding protein. It is expressed by hematopoietic cells, lymphoid cells and some subpopulations of myeloid cells. Ikaros is required for lymphocyte development and mediates B and T cell differentiation. It is thought that Ikaros regulates these processes by altering transcription of its target genes through chromatin remodeling of DNA promoter regions (Gowda et al. 2017). To regulate transcription, Ikaros forms homo- or heterodimers with other Ikaros family members such as Helios, Aiolos, Eos or Pegasus. This dimerization forms a complex which is capable of binding DNA and initiating its transcriptional activity (Merkenschlager 2010). This clone 4E9 has been used in immunocytochemistry experiments to examine Ikaros expression in cells incubated with cholesterol to examine how the transcription factor modulates cholesterol uptake (Loeper et al. 2008).

DNA-binding protein Ikaros, IK1, Ikaros family zinc finger protein 1, IKZF1, LYF1, Lymphoid transcription factor LyF-1, ZNFN1A1