Anti-IFT88 Antikörper (A83301)

Name: Anti-IFT88 Antibody
Brand: Antibodies.com
SKU: A83301
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab42497). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen IFT88 für ELISA, WB, IF und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-IFT88 Antibody

Beschreibung

Goat polyclonal antibody to IFT88.

Anwendungen

ELISA, WB, IF, Flow Cytometry

Verdünnungen

ELISA: 1:128,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human, Mouse

Immunogen

Synthetic peptide corresponding to the C terminal region of human IFT88.

Sequenz

C-KKRIDEDDFADEE

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

90 kDa

Geschätztes MW

90.4kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

D13S1056E, DAF19, hTg737, IFT88_HUMAN, Intraflagellar transport 88 homolog, Intraflagellar transport protein 88 homolog, MGC26259, Polaris homolog, Probe hTg737 (polycystic kidney disease, autosomal recessive), Recessive polycystic kidney disease protein Tg737 homolog, RP11-172H24.2, Tetratricopeptide repeat domain 10, Tetratricopeptide repeat protein 10, TG737, TPR repeat protein 10, TTC10

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Western Blot - Anti-IFT88 Antibody (A83301)

IFT88 expression in A549 (A) cell lysate and Mouse Testes (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-IFT88 Antibody (A83301) at 3µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-IFT88 Antibody (A83301)

IFT88 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IFT88 Antibody (A83301) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-IFT88 Antibody (A83301)

IFT88 expression in HepG2 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IFT88 Antibody (A83301) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-IFT88 Antibody (A83301)

IFT88 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in Tris/EDTA buffer, pH 9. Staining was performed with Anti-IFT88 Antibody (A83301) at 3µg/ml and revealed with horseradish peroxidase (HRP).