Anti-Iba1 Antikörper (A83222)

Name: Anti-Iba1 Antibody
Brand: Antibodies.com
SKU: A83222
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Novus (NBP1-06014). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen Iba1 für ELISA, Flow Cytometry, IF und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Stichworte:

Microglia Marker

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Polyklonal Kaninchen Antikörper gegen Iba1 für WB, ICC/IF und IHC.

Produktdetails

Produktname

Anti-Iba1 Antibody

Beschreibung

Goat polyclonal antibody to Iba1.

Anwendungen

ELISA, Flow Cytometry, IF, IHC

Verdünnungen

ELISA: 1:16,000, IHC: 2-4µg/ml, Flow Cytometry: 10µg/ml, IF: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human Iba1 (Isoform 1 and 3).

Sequenz

C-YEEKAREKEKP

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

AIF 1, AIF-1, Aif1, AIF1 protein, AIF1_HUMAN, Allograft inflammatory factor 1, Allograft inflammatory factor 1 splice variant G, allograft inflammatory factor-1 splice variant Hara-1, balloon angioplasty responsive transcription, BART 1, G1, G1 putative splice variant of allograft inflamatory factor 1, IBA 1, interferon gamma responsive transcript, Interferon responsive transcript 1, interferon responsive transcript factor 1, Ionized calcium binding adapter molecule 1, ionized calcium-binding adapter molecule, Ionized calcium-binding adapter molecule 1, IRT 1, IRT1, Microglia response factor, MRF1, Protein g1

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Immunohistochemistry - Anti-Iba1 Antibody (A83222)

Iba1 expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in Tris/EDTA buffer, pH 9. Staining was performed with Anti-Iba1 Antibody (A83222) at 4µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 2: Flow Cytometry - Anti-Iba1 Antibody (A83222)

Iba1 expression in K562 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Iba1 Antibody (A83222) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 3: Immunofluorescence - Anti-Iba1 Antibody (A83222)

Iba1 expression in CaCo-2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Iba1 Antibody (A83222) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.