Human sTNF RI ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sTNF RI in the samples. A monoclonal antibody specific for human sTNF RI has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for sTNF RI, and sTNF RI present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sTNF RI bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.08 pg/ml

Detektionsbereich

7.81 - 500 pg/ml

Wiederherstellung

105% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human sTNF RI.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

CD120a, FPF, MGC19588, p55, p55-R, p60, TBP1, TBPI, TNF R, TNF R55, TNF-R1, TNF-RI, TNFAR, TNFR-I, TNFR1, TNFR55, TNFR60, TNFRI, TNFRSF1a, TNR1A_HUMAN, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily, member 1A, Tumor necrosis factor receptor type 1, Tumor necrosis factor receptor type I, Tumor necrosis factor-binding protein 1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.