Human SCF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human SCF in the samples. A monoclonal antibody specific for human SCF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for SCF, and SCF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of SCF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.18 pg/ml

Detektionsbereich

31.25 - 2000 pg/ml

Wiederherstellung

95% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human SCF.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

C kit ligand, C-kit ligand, Ckit ligand , DCUA, DFNA69, DKFZp686F2250, familial progressive hyperpigmentation 2, FPH2, FPHH, KIT ligand, Kitl, KITLG, KL 1, KL1, Mast cell growth factor, MGF, MGF stem cell factor, SCF_HUMAN, SF, SHEP7, sKITLG, Soluble KIT ligand, Steel factor, steel, mouse, homolog of, Stem cell factor, Stem cell factor precursor

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.