The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Tissue homogenates, cell lysates and other biological fluids.

Sensitivität

0.054 ng/ml

Detektionsbereich

0.156 - 10 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

Cyclin A/CDK2 associated p19, Cyclin A/CDK2 associated protein p19, Cyclin-A/CDK2-associated protein p19, EMC19, MGC34403, OCP 2, OCP II, OCP II protein, OCP-2, OCP-II, OCP2, OCPII, Organ of Corti protein 2, Organ of Corti protein II, OTTHUMP00000159379, OTTHUMP00000159380, OTTHUMP00000228275, OTTHUMP00000228276, OTTHUMP00000228278, OTTHUMP00000228279, OTTHUMP00000228281, p19A, p19skp1, RNA polymerase II elongation factor like protein, RNA polymerase II elongation factor like protein OCP2, RNA polymerase II elongation factor-like protein, S phase kinase associated protein 1A, S phase kinase associated protein 1A p19A, S-phase kinase-associated protein 1, SIII, Skp 1, SKP 1A, Skp1, SKP1A, SKP1_HUMAN, TCEB1L, Transcription elongation factor B, Transcription Elongation Factor B 1 like, Transcription elongation factor B SIII polypeptide 1 like

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.