This RBP4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-RBP4 Antibody. Standards or samples are then added to the microtire plate wells and RBP4, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of RBP4 present in the sample, Anti-RBP4 Antibody (Biotin) is added to each well to "sandwich" the RBP4 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain RBP4 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivität

< 10 pg/ml

Detektionsbereich

312-20,000 pg/ml

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

OTTHUMP00000020114, OTTHUMP00000020115, OTTHUMP00000020116, Plasma retinol binding protein 4, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), PRBP, PRO2222, RBP, RDCCAS, RET4_HUMAN, Retinol binding protein 4, Retinol binding protein 4 interstitial, Retinol binding protein 4 plasma

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.