The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Tissue homogenates and other biological fluids.

Sensitivität

0.061 ng/ml

Detektionsbereich

0.156 - 10 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

193kDa vault protein, ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1, ADP ribosyltransferase like 1, ADP-ribosyltransferase diphtheria toxin-like 4, ADPRT L1, ADPRTL 1, ADPRTL1, ARTD4, H5 proline rich, I alpha I related, KIAA0177, Minor vault p193 protein, OTTHUMP00000042288, p193, PARP 4, PARP related, PARP related / IalphaI related H5 / proline rich, PARP4, PARPL, PH 5P, PH5P, Poly (ADP ribose) polymerase 4, Poly (ADP ribose) polymerase family member 4, Poly (ADP ribose) synthetase, Poly (ADP ribosyl) transferase like 1, Poly ADP ribose polymerase family member 4, Vault, Vault 3, Vault poly (ADP ribose) polymerase, Vault poly ADP ribose polymerase, Vault protein 193 kDa, Vault3, von Willebrand factor A domain containing 5C, VPARP, VWA5C

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.