Human PDE3B Kit Elisa (A271018)

Human PDE3B ELISA Kit

Human PDE3B ELISA Kit is a sandwich (quantitative) Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human PDE3B in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Sandwich (quantitative)

Human PDE3B ELISA Kit employs the Sandwich Enzyme Immunoassay (ELISA) technique for the quantitative measurement of human PDE3B in human. An antibody specific for PDE3B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the PDE3B present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-PDE3B Antibody, which binds the captured PDE3B present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of PDE3B captured in each well. The concentration of PDE3B can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colourmetric

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

26.35 pg/ml

78.1-5000 pg/ml

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Information online is representative. Always refer to the Product Manual inside the kit.

Store at +4°C. Do not use past expiration date!

5''-cyclic phosphodiesterase B, Adipocyte cAMP phosphodiesterase cGMP inhibited, AI847709, cAMP Phosphodiesterase, adipocyte, cGMP-inhibited, CGI-PDE B, CGIP1, CGIPDE B, CGIPDE1, cGMP inhibited 3 5 cyclic phosphodiesterase B, CGMP inhibited cAMP phophodiesterase, cGMP-inhibited 3'', Cyclic GMP inhibited phosphodiesterase B, Cyclic GMP-inhibited phosphodiesterase B, Cyclic nucleotide phosphodiesterase, HcGIP1, HcGIP1, rat, homolog of, PDE3B_HUMAN, Phosphodiesterase 3B, Phosphodiesterase 3B cGMP inhibited