Human P-Selectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sP-Selectin in the samples. A monoclonal antibody specific for human sP-Selectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for sP-Selectin, and sP-Selectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sP-Selectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

1.11 pg/ml

Detektionsbereich

78.13 - 5000 pg/ml

Wiederherstellung

96% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human P-Selectin.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

Antigen CD62, CD62, CD62 antigen like family member P, CD62 antigen-like family member P, CD62P, FLJ45155, GMP 140, GMP-140, GMP140, GMRP, Granule membrane protein 140, Granule membrane protein 140kDa, Granulocyte membrane protein, GRMP, LECAM 3, LECAM3, Leukocyte endothelial cell adhesion molecule 3 , Leukocyte-endothelial cell adhesion molecule 3, LYAM3_HUMAN, P selectin, PADGEM, Platelet activation dependent granule external membrane protein, Platelet activation dependent granule-external membrane protein, Platelet alpha granule membrane protein, PSEL, Selectin P, Selectin P (granule membrane protein 140kDa antigen CD62), Selectin P (granule membrane protein 140kDa, antigen CD62), SELP

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.