The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sensitivität

0.056 ng/ml

Detektionsbereich

0.156 - 10 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227, DF3 antigen, EMA, Episialin, H23 antigen, H23AG, KL 6, KL-6, KL6, Krebs von den Lungen-6, MAM 6, MAM6, MCD, MCKD, MCKD1, Medullary cystic kidney disease 1 (autosomal dominant), Medullary cystic kidney disease, autosomal dominant, MUC 1, MUC-1, MUC-1/SEC, MUC-1/X, MUC1, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, MUC1_HUMAN, Mucin 1 transmembrane, Mucin 1, cell surface associated, Mucin-1 subunit beta, Peanut reactive urinary mucin, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated epithelial membrane antigen, Tumor associated epithelial mucin, Tumor associated mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.